Effect of High 2-KLG Concentration on Expression of Pivotal Genes Involved in 2-KLG Synthesis in Gluconobacter oxydans WSH-003

Literature information

Author：

Hui Wan1 Zhen Kang1 Jianghua Li1 Jingwen Zhou1

Affiliations：

Key Laboratory of Industrial Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, China 214122

Year：

2016

Keywords：

Gluconobacter oxydans WSH-003; 2-keto-L-gulonic acid; pyrroloquinoline quinone; sorbitol dehydrogenase SDH; real-time PCR;

Abstract：

[Objective] To analyze the effect of high 2-keto-L-gulonic acid (2-KLG) concentration on important dehydrogenase, cofactor and transport proteins involved in 2-KLG synthesis. [Methods] First, the growth of Gluconobacter oxydans under high 2-KLG was observed. The real-time PCR was used to detect the expression of key sorbitol dehydrogenase gene sldAB, pyrroloquinoline quinone (PQQ) biosynthesis gene cluster pqqABCDE, and five genes encoding hypothetic PQQ transport proteins. [Results] According to results of the growth of G. oxydans under different 2-KLG concentration, 40, 80 and 120 g/L 2-KLG were decided to stimulate strains. Real-time PCR showed that PQQ biosynthesis genes pqqABCDE were not affected by high 2-KLG, but sorbitol dehydrogenase genes sldAB and part of genes encoding PQQ transport proteins were down-regulated under high 2-KLG stress. [Conclusion] The expression of sorbitol dehydrogenase genes was restrained by high 2-KLG, and PQQ transport was probably inhibited, but PQQ synthesis was not affected.